Effects of fishmeal substitution with <i>Hermetia illucens</i> L on the growth, metabolism and disease resistance of <i>Micropterus salmoides</i>

Identifying suitable substitutes for fishmeal has been one of the important issues aquaculture industry decades. Recently, black soldier fly, Hermetia illucens L (HI) become most promising alternative protein in aqua-diets. This study investigated effects substitution with fresh HI larvae on growth, serum and liver parameters, intestinal enzyme activity, histomorphology innate resistance to Aeromonas schubertii Largemouth bass. Three hundred seventy-five bass were randomly allocated into five groups fed different diet proportions instead as 0, 5, 10, 15 20% 49 days. Results showed that growth performance fish, including feed conversion ratio intake not affected by dietary treatments 5 10% HI. However, levels them are significantly increased Serum alanine aminotransferase content increased. The total fish increased, while glutathione peroxidase activity was decreased. In 5% HI, GSH-PX Intestinal amylase hepatic all unaffected, epithelial cells intact villi clearly visible. After days, it found reduced amount A. colonisation spleen at 48 h 72 after challenge. It concluded replacement did alter performance, improved antioxidant level disease